Journal: Cell reports

Article Title: A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit

doi: 10.1016/j.celrep.2023.113260

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The lentiviral doxycycline-inducible PINK1 expression vector, pLv-TetOn-FKBP-PINK1(110–581)-mNeonGreen ( Neo ), was created using the backbone from pLv-TetOn-MCS1-P2A-MCS2 ( Neo ) (Addgene, #89180).

Techniques: Recombinant, Bradford Assay, Mutagenesis, Plasmid Preparation, Microscopy, Cell Culture, Software, Sequencing

Journal: eLife

Article Title: On demand expression control of endogenous genes with DExCon, DExogron and LUXon reveals differential dynamics of Rab11 family members

doi: 10.7554/eLife.76651

Figure Lengend Snippet:

Article Snippet: Lentiviral pCDH-antiGFPnanobody-mCherry-Rab11a (overexpressing control) was cloned using pCDH-EF1-tagBFP-T2A-mycBirA*-Rab11a (previously prepared in our lab) via SpeI/SalI and XbaI/XhoI complementary cleavage sites. pMito-mCherry-FRB , a gift from Stephen Royle (Addgene plasmid #59352), was used as a template for generation of pMito-iRFP670-FRB (used with GFP-FKBP for knocksideways). pSH-EFIRES-P-AtAFB2 (Addgene plasmid #129715) , pCDH-EF1-tagBFP-T2A-mycBirA*-Rab11a, and mTagBFP2 were used to generate lentiviral pCDH-AtAFB2-mTagBFP2.

Techniques: Modification, Transfection, Construct, shRNA, Recombinant, Expressing, Plasmid Preparation, CRISPR, Knock-In, Labeling, Sequencing, Software, Invasion Assay

Journal: iScience

Article Title: Immunometabolic adaptation in monocytes underpins functional changes during pregnancy

doi: 10.1016/j.isci.2024.109779

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Article Snippet: CD195/CCR5 human antibody (clone REA245) , Miltenyi , Cat#130-117-356; RRID: AB_2733783.

Techniques: Recombinant, Adhesive, Sequencing, Modification, DC Protein Assay, Staining, Imaging, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Gene Expression, Software, Saline

Journal: Cell systems

Article Title: VISAGE Reveals a Targetable Mitotic Spindle Vulnerability in Cancer Cells

doi: 10.1016/j.cels.2019.05.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Phospho S10 histone H3 [3H10] EMD Millipore Cat#05-806; RRID: AB_310016 MTH1 [H-159] Santa Cruz Biotechnology Cat#sc-67291; RRID: AB_2153825 MTH1 [D6V4O] Cell Signaling Technologies Cat#43918; RRID: AB_2799252 Hemagglutinin tag HA.11 [16B12] Covance Cat#mms-101p; RRID: AB_2314672 Tubulin [YL1/2] Novus Biologicals Cat#NB600-506; RRID: AB_10078394 Pericentrin Abcam Cat#ab4448; RRID: AB_304461 GFP [3E6] Life Technologies Inc. Cat#A-11120; RRID: AB_221568 β-actin [AC-15] (mouse) Sigma Aldrich Cat#A5441; RRID: AB_476744 MAD2 Bethyl Labs Inc. Cat#A300-300A; RRID: AB_309443 Cleaved caspase-3 [C92-605] BD Pharmingen Cat#559565; RRID: AB_397274 Cleaved caspase-3 [5A1E] Cell Signaling Technologies Cat#9664; RRID: AB_2070042 β-actin (rabbit) Cell Signaling Technologies Cat#4967; RRID: AB_330288 Bacterial and Virus Strains Rosetta 2™ E. coli strain Novagen Cat#71402-3 Biological Samples None Chemicals, Peptides, and Recombinant Proteins DMEM Corning CellGro™ Cat#10-017-CV RPMI-1640 Gibco™ Cat#11875-093 EBM-2 Lonza Cat#CC-3156 EGM™−2 Bulletkit™ Lonza Cat#CC-4176 Fetal Bovine Serum (FBS) Seradigm Cat#1500-500 TH588 Thomas Helleday (Karolinska Institute) and Selleck Chemicals.

Techniques: Virus, Recombinant, Protease Inhibitor, Transfection, Expressing, Negative Control, Control, Software, CRISPR

Journal: Cell Reports

Article Title: Aryl hydrocarbon receptor activity downstream of IL-10 signaling is required to promote regulatory functions in human dendritic cells

doi: 10.1016/j.celrep.2023.112193

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Article Snippet: Anti human LAG3-PE , Miltenyi Biotec , Cat# 130-120-470;RRID: AB_2784078.

Techniques: Purification, Recombinant, Isolation, Ex Vivo, Microarray, In Vitro, Software, Sequencing